Eukaryotic Gene Expression Systems Workshop

International Conference on Structural Genomics

Toronto, Ontario, Canada

May 10th 2011

Organizers:

Stephen K. Burley, Eli Lilly and Company, San Diego, CA
Steven C. Almo, Albert Einstein College of Medicine, Bronx, NY

Workshop Objectives:

The goal of this workshop is to provide participants with a detailed understanding of the state-of-the-art for production of eukaryotic proteins destined for biochemical, biophysical, and structural analyses. The potential/limitations of bacteria and insect and mammalian cells and cell-free systems will be reviewed, with reference to experiences in various high-throughput structural genomics centers. In addition, there will be a focus on the development/exploitation of new expression strategies including enhanced host systems and technologies for production of multi-protein assemblies, which represent important targets for the next generation of structural biologists.

Confirmed Speakers/Affiliations/Titles:

• Stephen K. Burley
  NYSGXRC and Eli Lilly and Company, San Diego, CA
  
  What is the Real Effectiveness of E. coli for Expressing Eukaryotic Genes?



• Ron Seidel
  NYSGRC and Albert Einstein College of Medicine, Bronx, NY
  
  Refolding and Beyond: High Throughput Expression of Eukaryotic Proteins



• Susanne Gräslund
  SGC and Karolinska Institutet, Sweden
  
  High-Throughput Eukaryotic Protein Expression for Structural Genomics



• Scott A. Lesley
  JCSG and Genomics Institute of the Novartis Research Foundation, San Diego, CA
  
  Parallel Strategies of Mammalian Expression for Structural Genomics



• Yaeta Endo
  Cell-Free Science and Technology Research Center, Ehime University, Japan
  
  RIBOENGINE: Wheat Germ Cell-Free Protein Production System for Post-Genomic Research



• Imre Berger
  EMBL Grenoble, France
  
  Towards Eukaryotic Structural Complexomics

This workshop is generously sponsored by Genescript.