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Crystallography / Home Page

Team Members

  • John Walker Ph.D., Senior Research Associate, Macromolecular Crystallography
  • Wolfram Tempel, Ph.D., Senior Research Associate, Macromolecular Crystallography
  • Amy Wernimont, Ph.D., Research Associate, Macromolecular Crystallography
  • Aiping Dong M.Sc., X-ray Facility Manager

Select Publications

Wernimont AK, Amani M, Qiu W, Pizarro JC, Artz JD, Lin YH, Lew J, Hutchinson A, Hui R (2011) Structures of parasitic CDPK domains point to a common mechanism of activation. Proteins 79:803-20

Wernimont AK, Artz JD, Finerty P Jr, Lin YH, Amani M, Allali-Hassani A, Senisterra G, Vedadi M, Tempel W, Mackenzie F, Chau I, Lourido S, Sibley LD, Hui R. (2010) Structures of apicomplexan calcium-dependent protein kinases reveal mechanism of activation by calcium Nat Struct Mol Biol. 17:596-601

Himanen JP, Yermekbayeva L, Janes PW, Walker JR, Xu K, Atapattu L, Rajashankar KR, Mensinga A, Lackmann M, Nikolov DB, Dhe-Paganon S (2010) Architecture of Eph receptor clusters PNAS 107:10860-5

Tempel W, Wu H, Dombrovsky L, Zeng H, Loppnau P, Zhu H, Plotnikov AN, Bochkarev A (2009) An intact SAM-dependent methyltransferase fold is encoded by the human endothelin-converting enzyme-2 gene Proteins. 74:789-93

Wernimont A, Edwards A (2009) In situ proteolysis to generate crystals for structure determination: an update. PLoS One. 4:e5094

Tempel W, Tong Y, Dimov S, Bochkarev A, Park H (2008) First crystallographic models of human TBC domains in the context of a family-wide structural analysis. Proteins. 71:497-502

Avvakumov GV, Walker JR, Xue S, Li Y, Duan S, Bronner C, Arrowsmith CH, Dhe-Paganon S (2008) Structural basis for recognition of hemi-methylated DNA by the SRA domain of human UHRF1. Nature. 455:822-5

Bochkarev A, Tempel W. (2008) High throughput crystallography at SGC Toronto: an overview. Methods Mol Biol. 426:515-21

Dong A, Xu X, Edwards AM et al. (2007) In situ proteolysis for protein crystallization and structure determination. Nat Methods.41019-21

Allali-Hassani A, Pan PW, Dombrovski L, Najmanovich R, Tempel W, Dong A, Loppnau P, Martin F, Thornton J, Edwards AM, Bochkarev A, Plotnikov AN, Vedadi M, Arrowsmith CH. (2007): Structural and chemical profiling of the human cytosolic sulfotransferases. PLoS Biol., 5(2):e23.

Bochkareva E, Martynowski D, Seitova A, Bochkarev A. (2006): Structure of the origin-binding domain of simian virus 40 large T antigen bound to DNA. EMBO J., 25(24):5961-9.

Lunin V.V., Dobrovetsky E., Khutoreskaya G., Zhang R., Joachimiak A., Doyle D.A., Bochkarev A., Maguire M.E., Edwards A.M., Koth C.M. (2006): Crystal structure of the CorA Mg2+ transporter. Nature 440:833-837.

Bochkareva E., Kaustov L., Ayed A., Yi G.S., Lu Y., Pineda-Lucena A., Liao J.C.C., Okorokov A.L., Milner J., Arrowsmith C.H., Bochkarev A. (2005): Single-stranded DNA mimicry in the p53 transactivation domain interaction with Replication Protein A. Proc Natl Acad Sci USA. 102:15412-7

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